Objectives and scope InVet (Veterinary Research) is a journal whose objective is to disseminate scientific and academic works that address the issues inherent in the areas of knowledge of Veterinary Sciences: Basic Sciences, Animal Production, Preventive Medicine, Public Health and Bromatology, Animal Health and General Training. This publication is aimed at veterinarians, physicians, biologists, animal scientists, animal scientists, nutrition technicians, and all professionals related to animal health and production. Since 2024, the journal has adopted a continuous publication model and publishes one volume per year.
Facultad de Ciencias Veterinarias de la Universidad de Buenos Aires
Impact of Saccharomyces boulardii RC009 administration on clinical and microbiological parameters in Thoroughbred racehorses: a controlled study
This study evaluated the effect of Saccharomyces cerevisiae var. boulardii RC009 on body condition, coat quality, fecal score, and fecal microbiota profile in 25 Thoroughbred racehorses over 30 days. Animals were divided into a control group (CG) and a treated group (TG), the latter supplemented with 2x1010 CFU/day. In the TG, coat condition improved significantly: 53.8 % increased by one grade, and the remaining 46.2 % by two grades. Regarding faecal consistency, 58.3 % of the CG and 53.8% of the TG increased their score by one point. Microbiologically, TG showed a relative increase in the families Lactobacillaceae and Prevotellaceae, taxa associated with optimized digestion. The results indicate that S. boulardii RC009 supplementation positively impacted body condition, coat appearance, and faecal microbiota profile. These findings suggest that the probiotic favorably influences host physiology and microbial composition, supporting the need for further longitudinal studies with larger cohorts to further explore these mechanisms.
Copyright (c) 2026 Paola Alejandra Magnoli, Ana Laura Dávila, Laureano José Giordano, Julieta Luna, Ricardo Ludueña, Danilo Ceschin, Lilia René Cavaglieri https://creativecommons.org/licenses/by-nc-nd/4.0
Cytological evaluation of neutrophil infiltration in different uterine regions of sows destined for slaughter: implications for reproductive diagnosis
The aim of this study was to determine the neutrophilic polymorphonuclear infiltrate and the association between the portion of the uterus and the ovarian phase. Eighty-seven genital tracts were used from slaughtered sows. Samples were taken for cytology with an endocervical brush, on the body, distal horn, middle horn and proximal horn. The samples were stained with Romanovsky stain. Up to 200 cells were counted, considering endometrial cells and neutrophils. The statistical analysis was performed with a Poisson model and determined a higher count of neutrophilic polymorphonuclear in the body with respect to the horns (P≤0.01), but it was not associated with the ovarian phase (P=0.29). To determine the relationship between neutrophil count and ovarian phase, a mixed Poisson regression model was used. The neutrophilic polymorphonuclear count is higher in the follicular phase than in the luteal phase (P=0.11) and was only significant in the distal horn (P<0.01). It can be concluded that there is a variation in the number of neutrophils between portions of the uterus, independent of the estrous cycle. Cytology of the uterine body is a possible tool in live females for the diagnosis of endometritis and its implication in reproductive performance.
Copyright (c) 2026 Sofía Magalí Fages, Sara Inés Williams https://creativecommons.org/licenses/by-nc-nd/4.0
Structural and vascular alterations in placentas from cloned horses and their relationship with placental efficiency
The aim of this study was to describe the macroscopic and microscopic characteristics of placentas from cloned horses and to evaluate their placental efficiency compared with placentas from natural pregnancies. Samples were collected from recipient mares that delivered cloned foals (n=10) and from mares with natural pregnancies (n=10). A comprehensive structural (macroscopic and microscopic) analysis was performed, and placental weight, foal birth weight, and placental efficiency were recorded. In the control group, the allantoic surface appeared intact and bluish translucent, while the chorionic surface was reddish and velvety, with evident microcotyledons. In contrast, cloned placentas showed severe structural alterations, including reduction or absence of microcotyledons, amniotic thickening, fibrosis, hematomas, and signs of placental vasculopathy. Despite the viability of cloned foals, they exhibited lower birth weights and reduced placental efficiency, as cloned placentas were significantly heavier than those of controls. Histological analysis confirmed a chronic vasculopathy characterized by concentric perivascular collagen fibrosis, thickening of the tunica media, and luminal stenosis, suggesting decreased maternal–fetal blood flow. These macro- and microstructural alterations may reflect compensatory mechanisms associated with placental insufficiency.
Copyright (c) 2025 Mariana Rita Fiorimanti, Macarena Ferrario, María Fernanda Ortiz de Elguea, Keisy Gómez, Andrea Cristofolini, Rafael Audap-Soubie, Natalia Belén Pallarols Molinari, Alessandra Macchiavello Valdés, Mario Salvi, Cecilia Merkis https://creativecommons.org/licenses/by-nc-nd/4.0
XIV Jornadas de Jóvenes Investigadores 4 y 5 de junio de 2025 Universidad de Buenos Aires Facultad de Ciencias Veterinarias Buenos Aires - ARGENTINA
Universidad de Buenos Aires Facultad de Ciencias Veterinarias
Copyright (c) 2025 Universidad de Buenos Aires. Facultad de Ciencias Veterinarias https://creativecommons.org/licenses/by-nc-nd/4.0
Sustainability in equine production: application of environmental impact indices in Buenos Aires
The study evaluated the environmental impact indices proposed by Vaccaro et al.12 in seven sport horse production facilities located in Buenos Aires Province, Argentina. Based on surveys with producers and interviews with veterinarians, key variables were analyzed, including proximity to water bodies, manure and bedding management, veterinary supervision, diets, and water consumption. Results showed that 29% of the facilities were located less than 300 meters from water bodies, which increases the risk of water contamination due to nutrient runoff. Manure management practices ranged from systematic collection and reuse in orchards or nurseries to open-air deposition, leading to leachate generation and greenhouse gas emissions. Operations with frequent veterinary oversight and balanced diets exhibited lower environmental impacts, mainly due to reduced nutrient excretion. Water consumption for horse bathing varied widely depending on the intensity of sporting activities, highlighting the need for water efficiency strategies and improved infrastructure. The study concludes that the evaluated indices are useful as a diagnostic tool and that achieving sustainability in equine production requires environmental management plans tailored to each facility. These plans should include proper waste handling, responsible water use, and ongoing veterinary guidance.
Copyright (c) 2025 Mariana Vaccaro, Mariela Soto, José Alberto García Liñeiro, Alejandra Volpedo https://creativecommons.org/licenses/by-nc-nd/4.0
I International Conference on Veterinary Epidemiology
Primeras Jornadas Internacionales de Epidemiología Veterinaria 7 y 8 de noviembre 2024 Universidad de Buenos Aires Facultad de Ciencias Veterinarias Buenos Aires - ARGENTINA
International Conference on Veterinary Epidemiology
Copyright (c) 2025 Universidad de Buenos Aires Facultad de Ciencias Veterinarias Buenos Aires, Argentina. https://creativecommons.org/licenses/by-nc-nd/4.0
El desmantelamiento del sistema científico argentino: entre el ajuste fiscal y el “cientificidio”
Copyright (c) 2025 Alejo Pérez Carrera, Daniel Marcelo Lombardo https://creativecommons.org/licenses/by-nc-nd/4.0
Mechanical behavior of the isolated equine metatarsal bone III during a flexion test with acoustic emission monitoring
The objective of this study was to determine the mechanical behavior of the equine Metatarsal bone III under a three-point bending test and its relationship with bone morphology and the appearance of acoustic emission events, providing biomechanical knowledge applicable to veterinary medicine. The equine Metatarsal bone III of 20 Creole crossbreed horses were studied, divided into two age groups: animals from 1.5 to 2 years old (G1) and from 2.5 to 4.5 years old (G2). A transverse osteotomy was performed on the left bones in the middle of the diaphysis, and the following measurements were made: cortical thickness, cortical area, and medullary area. The right bones were subjected to a three-point bending test, while acoustic emission monitoring was performed simultaneously. The study variables were subjected to statistical analyses of correlation, regression, and variance (p≤0.05). It was observed that the metatarsals III of group G2 showed greater resistance than those of group G1 (p=0.016) furthermore, the Acoustic Emission hits increased as bone strength increased. This monitoring allows for the assessment of bone damage before to the complete fractures in bones subjected to stress.
Copyright (c) 2025 Rita Cecilia Fioretti, Rosana Moine, Pablo Varela, Rodrigo De Prada, Mario Salvi, Matías Varela, Horacio Mouguelar, Maximiliano Cancino, Silvana Gonzalez Sanchez, Yanina Andino, Ana Cecilia Liaudat https://creativecommons.org/licenses/by-nc-nd/4.0
Effect of the use of acetylcysteine on the reduction of the percentage of neutrophil polymorphonuclear cells present in subclinical endometritis of dairy cattle diagnosed with the cytobrush technique
The objective of the present study was to evaluate the effect of N-acetylcysteine (NAC) administration as a pretreatment prior to antibiotic therapy in the control of subclinical endometritis (SE). Endometrial mucosa samples were collected from 14 housed Holstein Friesian cows from the Majes Irrigation Area, Caylloma, Arequipa, Peru. The animals were between their first and fourth lactation, with a body condition score (BCS) of 4. A cervical–uterine cytological brush was used for Papanicolaou sampling. Smears were prepared and submitted to the laboratory, stained using the Diff-Quick technique, and examined under bright-field microscopy for the count and calculation of the percentage of polymorphonuclear neutrophils (%PMN-N). SE was confirmed when %PMN-N was ≥ 5%. The brushes were transported in tubes containing Amies transport medium for subsequent aerobic culture on MacConkey agar, Mueller–Hinton agar, blood agar base, and Chromagar, with bacterial identification from the endometrial mucosa performed through Gram staining and biochemical tests. Antibiograms were carried out according to the bacteria identified. Of the 14 cows sampled, 11 showed %PMN-N values above the 5% threshold and were classified as SE-positive, with those below this value excluded from further analysis. Two groups were formed: T-NAC (n = 5) and nT-NAC (n = 5). The T-NAC group received an intrauterine infusion of 40 cm3 of 2% NAC (Fluimucil® 300 mg/3 mL) for three consecutive days, starting 72 hours after sampling, while the nT-NAC group served as a control (no NAC treatment). Endometrial bacteria were isolated in four cows, identified as Staphylococcus epidermidis, Staphylococcus aureus, and Escherichia coli. Twelve hours after NAC administration, differentiated intrauterine antibiotic treatment was applied to both groups according to the bacterial species identified. Seven days after the first sampling, vaginal cytology was repeated to reassess %PMN-N. Data were analyzed using InfoStat 2020 software. Summary and dispersion measures were calculated; for the total animals evaluated for PMN-N (n = 14), the median was 9.50 with a standard error (SE) of 1.21. Paired-sample inference was performed using the Student’s t-test for both treatment and control groups with a two-tailed contrast, yielding a statistically significant effect for the T-NAC group (p < 0.05) and a non-significant result for the nT-NAC group (p > 0.05). In conclusion, intrauterine administration of N-acetylcysteine in cows with subclinical endometritis significantly reduces %PMN-N.
Copyright (c) 2025 Fernando A. Fernandez Fernandez, Kristel Pacheco, Hasan Icen, Servet Badenkyran, Víctor Pacheco, Daniel Marcelo Lombardo https://creativecommons.org/licenses/by-nc-nd/4.0
Residues of organic contaminants in honey from four villages in Buenos Aires province
The province of Buenos Aires is the leading honey producer in Argentina. The quality of the honey is a critical factor in its commercialization, particularly for export; therefore, the potential contamination by pesticides is a significant concern in honey production. In this context, a survey of honey samples from this province was conducted, identifying the presence of 36 pesticide residues and/or their metabolites. The objective of this study was to analyze the presence of organic xenobiotics in honey and to compare the results based on the predominant anthropogenic activities in each surveyed area. The results for all pesticide residues and their metabolites were found to be below the detection limit (LOD) of the corresponding analytical method, ensuring the purity of the honey in relation to these organic contaminants.
Copyright (c) 2025 Natalia Yoshida, Diego Grassi, Gustavo Thompson, Carlos Ojeda, Alejo Perez Carrera https://creativecommons.org/licenses/by-nc-nd/4.0
Obtención de cachemira por administración de extracto natural con mimosina y malla de contención como método de cosecha
Cashmere is obtained through combing, which, due to the variation in shedding patterns among animals, requires up to three repetitions and is difficult to optimize. Shearing must be done before the spring molt, and the goats are sensitive to low temperatures. A method of synchronized, uniform harvesting with animal welfare is needed. To modify the molting of cashmere goats, there are no available physiological techniques, except for the use of mimosine, which must be applied according to the seasonal follicular cycle of the skin, with appropriate means. The objective was to analyze the method of administering Leucaena leucocephala extract with mimosine and a covering mesh for cashmere production. Creole adult goats at 35° S, in a semi-extensive system in La Pampa, Argentina, were treated with a single dose of 500 mg of mimosine/kg of live weight, orally, and with a containment mesh. In this way, mimosine has an effect lasting no more than 24 hours, causing total shedding of fine fiber without significantly affecting the coarse fiber; no clinically detectable toxic effects were observed. The mesh is fundamental for the viability of the method, and the obtained fiber has textile suitability and superior discard behavior compared to other harvesting methods.
Copyright (c) 2025 Melina Castillo, María Bettina Gómez, Dante Alberto Cerutti, Pedro Vicente Palermo, María Flavia Castillo, Silvia Marina Ardoino, Alejandro Prieto, Michel Victor Huber Hick, Eduardo Narciso Frank https://creativecommons.org/licenses/by-nc-nd/4.0
SARS-CoV-2 in dogs: gamma variant and its transmission from humans in Ciudad de Buenos Aires, Argentina
This article explores SARS-CoV-2 infection in dogs that have had close contact with humans diagnosed with COVID in Argentina. Affirmative molecular identification of the virus was achieved through RT-PCR analysis of oropharyngeal and rectal swabs from the dog as well as a nasal swab from the guardian. Positive results for the dog were confirmed through a LAMP/CRISPR assay and real time PCR. SARS-CoV-2 genomic RNA was successfully obtained from these samples, and the analysis of a partial sequence of the spike gene (ranging from amino acid 428 to 750) allowed us to classify both genomic sequences as Gamma variants, with a single amino acid variation in position 726. We evaluated the specific humoral immune response of the dog by an indirect ELISA using the Argentinian kit COVID AR IgG ELISA test adapted for domestic dogs, and pseudo-neutralization conducted with SARS-CoV-2 pseudotyped particles. We also evaluated the specific cellular immune response of the dog through a lymphoproliferation assay, using purified spike protein as specific stimulus. This work provides evidence of the human-to-dog transmission of SARS-CoV-2 in Argentina, underscoring the One Health concept and reinforcing surveillance methods in pets.
Copyright (c) 2025 Marcelo Miragaya, Ana Jolly, Andrea Carrizo, Gabriel Cicuttin, Diego Fernando Eiras, Mariana Bertuzzi, Ana María Jar, María Mora Gonzalez Lopez Ledesma, Andrea Vanesa Gamarnik, Franco Lucero Arteaga, Danilo Bucafusco, Gabriel Capitelli, Silvia Leonor Mundo https://creativecommons.org/licenses/by-nc-nd/4.0
Detection of Salmonella spp. and enterobacterias in egg for consumption from cage-free systems
The presence of Salmonella spp. and the Enterobacteria count were studied in eggs from hens housed in cage-free systems on 30 farms in the province of Buenos Aires and southern Santa Fe. The analyses carried out to detect the presence of Salmonella spp., both on the shell and inside the egg, were negative. Regarding the Enterobacteria count, they were isolated in most of the eggs with colony count values within the permitted range, except for 4 % of the farms where higher values were detected. In these cases, it was recommended to improve biosecurity measures that also consider the safety of the eggs. These microorganisms can not only cause economic losses in poultry production, but also represent a risk to public health. The results indicate that if the cage-free production system incorporates good poultry practices, the eggs produced are of excellent microbiological quality.
Copyright (c) 2025 Zulma Canet, Olga Riva, Jose Daniel Rodriguez, Martin Andersen https://creativecommons.org/licenses/by-nc-nd/4.0
Bone remodelling: age-related changes in markers of bone formation and resorption associated with falling oestrogen levels in female rats
Biochemical markers identify the level of bone remodelling. The aim was to determine the levels of CTX, OCN and the OCN/CTX ratio as a function of age and to assess changes due to ovariectomy-induced oestrogen deficiency (OVX) in female Wistar rats. OCN and CTX were assessed and the OCN/CTX ratio was calculated in newborn, 21, 45, 60, 90, 105 and 150-day-old animals (n=8/group). The percentage change (%) from the newborn level was determined. OVX and SHAM rats were fed either Commercial diet or AIN-93M. OVX was performed at 45 days of age and proximal tibial BMD change (by DEXA) was assessed at 90 days in rats fed AIN-93M. OCN and CTX levels and OCN/CTX ratio were compared between the two groups at 90 and 105 days of age. OCN increased and CTX showed progressive decreases with age, stabilising at 90 days. The OCN/CTX ratio peaked at 90 days and decreased thereafter. OVX increased OCN in rats fed AIN-93M and did not vary with commercial diet; CTX increased with both diets. OVX decreased proximal tibia BMD relative to SHAM. Conclusions: Markers varied with age and the increase by OVX was diet-dependent, highlighting the importance of calcium, phosphorus and their relationship for bone health. Absolute values of OCN and CTX would allow establishing reference levels in female rats fed with commercial diet.
Copyright (c) 2025 Estefanía Magalí Zeni Coronel, Gabriel Bryk, Marina Soledad Bonanno, Mariana Seijo, Susana Noemí Zeni https://creativecommons.org/licenses/by-nc-nd/4.0
XIII Jornadas de Jóvenes Investigadores 28 de junio de 2024 Universidad de Buenos Aires Facultad de Ciencias Veterinarias Buenos Aires - ARGENTINA
Copyright (c) 2024 Universidad de Buenos Aires. Facultad de Ciencias Veterinarias https://creativecommons.org/licenses/by-nc-nd/4.0
Chitosan hydrogels crosslinked with glyoxal as controlled drug release systems
Chitosan hydrogels have garnered attention as transdermal drug delivery systems due to their ability to facilitate controlled release of bioactive compounds, achieving systemic therapeutic effects. In this study, we synthesized and characterized chitosan hydrogels crosslinked with glyoxal, targeting their potential as controlled drug release systems in animal production. The hydrogels were extensively characterized using Fourier Transform Infrared Spectroscopy to identify functional groups and confirm the successful incorporation of insulin. Swelling studies demonstrated a maximum swelling ratio of 4155 % at acidic pH, indicating significant water uptake and potential for drug delivery applications. Biocompatibility assessments, including hemolysis and MTT assays, revealed a hemolysis percentage below 10 % and cell viability exceeding 95 % after 24 and 48 hours, underscoring the hydrogels’ safety profile. Insulin incorporation efficiency was 95.99 %, with a sustained release profile reaching a maximum of 13.19 % at 27 hours. These findings suggest that chitosan-based hydrogels crosslinked with glyoxal hold promise as effective platforms for slow or sustained transdermal drug delivery, particularly for therapeutic agents requiring prolonged release.
Copyright (c) 2025 Carolina Flores Bracamonte, Luján Pedraza, Fabrisio Alustiza, Andrea Bozzo, César Barbero, Romina Bellingeri, Maria Molina https://creativecommons.org/licenses/by-nc-nd/4.0
Microbiological study of effluents from artisanal cheese factories
In Tandil (Buenos Aires province) there are artisanal cheese factories, which are mostly located in rural areas. Seven cheese factories were selected, and sampling was carried out for 4 years to evaluate the microbiological quality of their effluents. Different ways of effluent disposal were identified: by direct discharge to the environment, or with a system of pools or stabilization lagoons. A total of 41 samples were microbiologically analyzed and the contents of thermotolerant coliforms were compared to the limits of the regulations by the Water Authority of the province of Buenos Aires. The results showed that 50% of the samples exceeded the required limit of thermotolerant coliforms. Escherichia coli, Enterococcus spp., and other enteric bacteria were also found in some of the samples. The problems encountered in the discharges were related to the lack of adequate advice to adopt efficient treatment systems and also to the cost involved in their implementation.
Copyright (c) 2025 Anahi Elvira Tabera, Guido Buccella, Nicolás Eloy Cisneros Basualdo, Corina Iris Rodríguez, Alejandra Krüger https://creativecommons.org/licenses/by-nc-nd/4.0
Dynamics of the structure and composition of Saccharomyces cerevisiae RC016 cell wall subjected to gastrointestinal conditions
The yeast Saccharomyces cerevisiae is a model organism, characterized by being a most valuable species for a variety of industrial applications. Between the applications of S. cerevisiae is its use as a livestock feed decontamination strategy, sequestering the mycotoxin molecule by adsorption, reducing the bioavailability from the digestive tract and preventing the AFB1 absorption through the intestine. The S. cerevisiae RC016 strain isolated from pig gut, showed biological control properties against Aspergillus parasiticus, Fusarium graminearum and Aspergillus carbonarium, was able to adsorb AFB1 promoted beneficial properties to the host with the absence of cytotoxicity and genotoxicity and was able to survive under gastrointestinal tract (GIT) conditions. Is important to deepen the knowledge of the morphological and ultrastructural characteristics of the yeast cell wall that allow the adsorption of mycotoxin. The objective was to evaluate the dynamic of the Saccharomyces cerevisiae RC016 cell wall under gastrointestinal (GIT) through different microscopic techniques. Calcofluor white technique, transmission electron microscopy, scanning electron microscopy, morphometric analysis and fluorescence intensity expression analysis were used. The cell wall thickness as well as the β-glucan-chitin expression were significantly higher in the gastric solution. In previous studies, our research grouphas determined that S. cerevisiae RC016 adsorbs the higher levels of aflatoxin B1 in gastric juice. In the present study the cells preserved the external and internal architecture in gastric solution while in the intestinal solution the three-dimensional morphology and ultrastructural conformation were notably altered. The yeast in gastric solution maintained its ultrastructural morphology, showed an important cell wall thickening and had high β-glucan-chitin levels. Calcofluor white staining, a specific and low-cost technique, allowed a later morphometric-densitometric analysis of the cell wall. It is important to have a technique that differentially determine the portion of the yeast cell wall that allows the adsorption. The use of S. cerevisiae RC016 as bio-detoxifier would be a promising and economical strategy to reduce the exposure of livestock to mycotoxins.
Copyright (c) 2024 Andrea Lorena Cristofolini, Analía Fochesato, Mariana Fiorimanti, Alexis Alfonso, Carlos Gomez, Valeria Poloni, Keisy Gómez, Cecilia Merkis, Lilia Cavaglieri https://creativecommons.org/licenses/by-nc-nd/4.0
Vertical mobility of arsenic in a sandy soil superficially amended with biosolids or manure
The accumulation of excreta in intensive livestock systems or biosolids in large cities constitutes a serious ecological and economic problem. The presence of arsenic in drinking water causes this element to be found in excreta. Given its toxicity even at relatively low concentrations, the objective of this work was to study the vertical mobility of arsenic (As) in columns of a disturbed sandy soil, superficially amended with manure or biosolids (treatments E and B respectively). After 30 days, the maximum availability of As was observed between 3.5 and 7 cm depth in E, while the availability of As in B was below the quantification limit in all the studied strata. In leachates, the As concentration was significantly higher in E at the end of the trial, compared to B or control. This mobilization may be attributed to the higher concentration of phosphorus or to the soluble organic matter generated by manure throughout the test, facilitating the vertical migration of As.
Copyright (c) 2024 Silvana Irene Torri, Alejo Pérez Carrera https://creativecommons.org/licenses/by-nc-nd/4.0
Renal hemodynamic parameters by Doppler ultrasound in pre- and post-anesthetic stages in healthy domestic canines
In the present work, the variability of Resistance Index, Pulsatility Index, Peak Systolic Velocity and End Diastolic Velocity of renal and interlobar arteries was analyzed in pre- and post-anesthetic stages in healthy bitches undergoing ovaryhysterectomy. The sample consisted of 24 bitches, > 6 months, arranged in 3 groups according to size: < 8 kg, between 8-20 kg and > 20 kg. Pulsed Spectral Doppler was performed on the cranial, middle and caudal renal and interlobar arteries in 3 times: 24 hours before the anestheticsurgical procedure, 24 hours and 72 hours after completion of the conventional hysterectomy. The end diastolic velocity presented differences between <8 kg and >20 kg (p=0.03) in renal artery and sizetime interaction (p=0.015), lower in <20 kg compared to >20 kg in interlobar arteries (p≤0.0001). The resistance index showed size-time interaction (p=0.0003), in large sizes it was lower than in small and medium sizes 24 hours after closing the vaporizer. The pulsatility index presented analogous behavior. The hemodynamic effects, due to hypotension, increase when size decreases and are more evident in female dogs <8kg. The Doppler variables of interlobar arteries show greater variability than those recorded in the renal artery.
Copyright (c) 2024 María Carolina Miguel, Fernando J Río, Marisa E Giménez, Natalia Cazaux, Milton C Gorra Vega, Diego Hernández, Cintia Calvo, Lourdes Viqueira Sánchez, Verónica Véspoli Pucheu, Gabriela Volpi Lagreca, Alberto Meder https://creativecommons.org/licenses/by-nc-nd/4.0
Characterization of fetal placental collagen in nutritionally restricted goats during the prepubertal stage
The objective of the work was to identify, through optical microscopy, transmission electron microscopy and the picrosirius red technique, the presence and disposition of collagen in term fetal placentas, coming from a nutritional restriction model. Samples of placental cotyledons were taken for analysis of tissue and vascular structure using Masson's trichrome staining and for analysis of collagen morphology and arrangement using transmission electron microscopy and picrosirius red. In restricted fetal placentas, larger vessels were observed in relation to control placentas. In them, green and orange collagen fibres predominated, thin and thick respectively. In the control placental samples, orange and yellow, thick and intermediate fibres were detected, respectively. Nutritionally restricted females expressed adequacy in the development of the placental vascular network, and revealed changes in the expression and arrangement of collagen fibres, particularly the thicker fibers, essential for maintaining placental resistance and integrity throughout gestation. These morphological differences between treatments demonstrated that female goats are capable of facing adverse environments through metabolic and physiological adaptation mechanisms.
Copyright (c) 2024 Keisy Pabla Gomez, Mariana Rita Fiorimanti, Andrea Lorena Cristofolini, Anabella Benzoni, Mauricio Luján, Oscar Luján, Claudio Gustavo Barbeito, Cecilia Inés Merkis https://creativecommons.org/licenses/by-nc-nd/4.0
Serological surveillance of the prevalence of Ehrlichia canis in the southern departments of Santa Fe province, Argentina
Canine ehrlichiosis is a disease that affects canines, has a worldwide distribution and is transmitted by Rhipicephalus sanguineus sensu lato ticks. It was officially described, for the first time in Argentina, in 2013. To date, it has been recorded in dogs from numerous cities and provinces. The objective of this work was to report the seroprevalence of Ehrlichia canis along with the hematological alterations found in patients from the Rosario, San Lorenzo, Constitución and Caseros departments of the province of Santa Fe, in the period 2017-2019. There are different clinical manifestations of the disease and with them associated hematological alterations. In the present work, a retrospective analysis was carried out on the dogs that were indicated for a serological analysis of Ehrlichia canis together with a blood count. The results obtained showed that of the 468 dogs studied, 50.4 % were positive. No association was observed between sex, age or race. 60.5 % of the positive cases presented some hematological alteration, among the most frequent being anemia, leukocytosis and thrombocytopenia. The blood count is a valuable tool to help the clinician in the diagnosis and treatment of patients with Ehlichia canis, since it often presents itself as a clinical challenge.
Copyright (c) 2024 Cora Colla, Jesica Ciminari, Melina Beatriz Gines, Mario Germán Bartolomeo https://creativecommons.org/licenses/by-nc-nd/4.0
Instituto de Investigaciones Clínicas Veterinarias - FCV UBA August 31 - September 1, 2023
Instituto de Investigaciones Clínicas Veterinarias FCV UBA
Copyright (c) 2023 InVet (Veterinary Research)
XII Jornadas de Jóvenes Investigadores 8 y 9 de junio de 2023 Facultad de Ciencias Veterinarias - Universidad de Buenos Aires Buenos Aires – ARGENTINA
Facultad de Ciencias Veterinarias Universidad de Buenos Aires
The protection against goat abortion by the anti-brucella primary vaccination, the challenge of revaccination
Goat brucellosis, by Brucella melitensis, is a zoonotic-endemic disease in eight provinces of Argentina. The National Animal Health Service (SENASA) urged the local governments to set up a control program that involves a conjunctival Rev-1 vaccination, every two years, of all goat populations independent of the age and sex of the animals. The vaccine posse the restriction not to be applied in pregnant goats because occasionally induce abortion. Practical evidence of herds vaccination, not validated by controlled experiences, showed that with revaccination pregnant goats did not abort, but were protected against it and even with further vaccinations. Therefore, the objective of the trial was to assess the effect of conjunctival Rev-1 vaccination of pre-immunized goats when were revaccinated during the higher susceptibility period to the abortion. Twenty-two, brucellosis-free Boer goats were randomly assigned to either yellow or red groups, according to the colour of the ear tag (gA n=11; gR n= 11). The natural mating lasted 60 days and the pregnancy percentage was 82 (gA) and 91 % (gR), respectively. The gA received two vaccine doses, at 20 days pre-mating and at 95 days from the beginning of mating, whereas gR received just one dose at 98 days of the beginning of mating. Blood samples were taken at six-time points to assess the immune response and microbiology samples were also taken at birth/abortus and five days thereafter. The microbiology samples were cultured at both Farrell and CITA medium, and every colony morphologically compatible with Brucella was validated by PCR (Multiplex PCR, Bruce-ladder). All the goats were serologically positive at 14 days post-inoculation, but the gR maintained a significant (p<0,05) number of goats positive at 100 days post-birth/abortus. The gA goats gave birth on time and the vaginal swabs were bacteriology negative at both parturition and five days thereafter, whereas the vaginal swabs of the gR were positive at both times. In conclusion, the pre-mating vacination against brucellosis prevented significantly (p<0,0001) both the abortion and the infections of the goats when they were revaccinated with the vaccine strain during the gestation.
Correlation of fibroblast growth factor receptor, platelet-derived growth factor receptor and cyclooxygenase-2 expression with neoplastic cell proliferation and vascularization in feline vaccine-associated sarcomas
Feline inoculation site-associated sarcomas are mesenchymal neoplasms that develop at sites of inoculation of substances. In this work, the immunohistochemical expression of fibroblast growth factors-2 and platelet-derived-B growth factors and their receptors was determined in feline sarcomas associated or not associated with inoculation sites. Both types of sarcomas expressed these growth factors and their receptors. In feline sarcomas associated with inoculation, cell proliferation positively correlated with vascularization and with the expression of both growth factors. In addition, fibroblasts growth factor correlated with the degree of anaplasia, whereas the number of vessels per field with COX-2. On the other hand, the degree of anaplasia was positively correlated with cyclooxygenase-2 enzyme. The carcinogenesis of different tumors is associated with the overexpression of these growth factors. Our results could justify the use of inhibitors of these receptors to reduce proliferation in this type of feline sarcoma; the degree of anaplasia could be of predictive value for sensitivity to tyrosine kinase or cyclooxygenase-2 inhibitors.
Copyright (c) 2024 InVet (Veterinary Research)
Effect of a commercial additive based on bioactive compounds (carvacrol, cinnamaldehyde, capsicum and cúrcuma) on productive performance and intestinal health in broiler chickens
This preliminary study aimed to evaluate the effect of a commercial additive based on bioactive compounds on the productive parameters and intestinal health in broiler chickens. A total of 100 one-day-old Cobb birds were used, and at 7 days were weighed and randomly selected into 2 treatment groups with 10 replicates/5 birds per replicate. During the experimental period (48 days), the broiler chickens received the starter and finisher diet. Treatment (T) T1: basal diet (BD - Control); T2: BD + commercial additive (100g/T). The histomorphometric parameters of the intestine, along with volatile fatty acids concentration and counts of lactic and enteric bacteria, were determined. At week 21, the addition of commercial additive resulted in significant differences in weekly live weight gain compared to T1 (p <0.05). The addition of commercial additive showed a positive trend in productive parameters and obtained a higher productivity index. The levels of volatile fatty acids and the count of lactic bacteria were found to be higher in T2 as compared to the control (p < 0.05). In conclusion, supplementation with the commercial additive showed an increasing trend in productive parameters, positively modulated intestinal health in broiler chickens, at least at the dose and with the number of animals used, constituting a possible alternative to replacing antibiotics as promoters of growth. Based on these results, it is proposed to carry out an in vivo trial with a higher number of animals and compare the efficiency with the inclusion of antibiotics as growth promoters.
Pharmacokinetics of amoxicillin and milk passage during lactation in goats
The aims of the present study were to describe the pharmacokinetic profile and milk concentrations of a slow release formulation of amoxicillin administered intramuscularly in goats at different times of lactation, and to determine the PK/PD ratio value. Five healthy lactating goats in their third lactation at 25-30 (peak milk yield) and 150–155 (end of lactation) days postpartum were used. At each experience 20 mg/kg intramuscular amoxicillin was administered. Blood and milk samples were collected at predetermined time and drug concentrations were determined by microbiological assay. In both periods plasma pharmacokinetic parameters were calculated using compartimental analysis. The pharmacokinetic profile was similar in both lactation moments. No differences were detected in the pharmacokinetic parameters or PK/PD values between treatments. Amoxicillin concentrations were not detected in milk. Our results suggest that, based on sensitivity test results, the dose used in this study could be administered every 48 hours, at both times studied for the treatment of infections caused by sensitive microorganisms with MIC ≤ 0.188 μg/mL. For less sensitive bacteria (MIC ≤ 0.25 μg/mL) the dosing interval should be longer during the peak milk yield (48 hours) than at the end of the lactation (22 hours). For microorganisms with MIC ≥ 0.5 μg/mL the serum concentrations achieved show that this formulation should not be used as a depot formulation.
Use of ketoprofen by systemic and local routes in orchidectomy with elastrator in lambs
Non-steroidal anti-inflammatory drugs are widely used in veterinary medicine for small animals because of their therapeutic effects, and these results cannot be extrapolated to the clinic of large animals because of the difference between these species. The objective of this study was to evaluate how these anti-inflammatory may influence in the orchidectomy with elastomers in lambs. Twenty-six male lambs were randomly divided into four groups: A control without castration; B castration without the use of ketoprofen; C castration with local administration of ketoprofen; D castration with intramuscular administration of ketoprofen. Ketoprofen was used at a dose of 3 mg/kg. Rectal temperature, behavioral attitudes correlated with pain, weight and reactions to the scrotal palpation were recorded in order to evaluate if there were significant differences with the use of ketoprofen. The results obtained showed greater painful attitudes in groups B and D than A and C, greater weight gain in groups A and C compared to D and B, and the decreasing order of painful reactions to scrotal palpation was B, D, C, A. The use of ketoprofen in orchidectomy with an elastomer would benefit animal welfare as it decreases pain and improves weight gain in lambs.